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Probe domestic high frequency needle JKT-50L with 3 probes
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Test - uppercase JPG - memory 146K
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EL-C1600N100013-B
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Kaixin micro test
ELISA is a highly sensitive, specific, and reproducible experimental diagnostic method. It is ideal for large-scale screening tests due to its stable reagents, easy storage, simple operation, and objective results. ELISA can be used for both qualitative and quantitative analysis, even when dealing with small sample sizes. It has become widely applied in fields such as microbiology, parasitology, oncology, and cytokine research.
The precision of an ELISA kit is typically measured by the coefficient of variation (CV) within a batch, which should generally be below 15%. For quantitative assays, it's also important to evaluate the linear range of the detection system.
High CV values in ELISA plates can often be traced back to several factors. One common issue is improper washing or handling of the coated plate during the addition of standards, samples, or reagents. Be careful not to scratch the well surfaces while performing these steps.
Ensure that the sample is well mixed before loading to avoid uneven distribution. A homogeneous sample is essential for accurate results.
Edge effects can occur if the plate is incubated in areas with temperature fluctuations, such as near vents or windows. To minimize this, it’s recommended to place the ELISA plate on a 400 rpm shaker and cover it with a plate seal.
Avoid stacking plates during incubation, as this can cause inconsistent temperature and humidity exposure across wells.
Pipetting errors are another common source of variability. Make sure the pipette is properly calibrated and used correctly to ensure accurate volume delivery.
Inadequate or skipped washing steps can significantly increase the CV value. Do not reduce the volume of the washing solution or shorten the time—leave the solution in each well for 15–30 seconds to ensure thorough removal of unbound substances.
Shanghai Jinma Bio offers three primary methods depending on the testing purpose and procedure:
1. The sandwich method is commonly used for antigen detection. It involves two monoclonal antibodies that recognize different epitopes on the target antigen. This method works best for bivalent or larger antigens and cannot detect small molecule haptens.
2. The indirect method is used for antibody detection. In this approach, an enzyme-labeled secondary antibody is used to detect the primary antibody that has bound to a solid-phase antigen.
3. The competitive method is suitable for detecting antigens or small molecule haptens. The tested antigen competes with the enzyme-labeled antigen for binding to the solid-phase antibody. The more antigen present, the less enzyme label is bound, resulting in a lighter color. This creates an inverse relationship between antigen concentration and signal intensity.
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