Shanghai ancient flower detailed description: ELISA kit OD value is not normal - Database & Sql Blog Articles

ELISA is a widely used technique in biological and medical research, but it can be quite complex due to the multiple steps involved and the various factors that can affect the reaction. As a result, many issues may arise during the experiment. However, most of these problems are avoidable with proper technique and attention to detail. Recently, a customer asked us about abnormal OD values—what they mean and what could cause them. Here’s a detailed explanation from Shanghai Guduo Biotechnology.

Elisa kit

If you're experiencing an unusual OD value, here are some common causes and solutions:

1. The kit wasn't properly warmed up. Solution: Make sure the kit is brought to room temperature (25°C) before use.

2. Temperature control was not accurate. Solution: Always maintain the correct incubation temperature as specified by the kit instructions.

3. Incubation time was not precise. Solution: Follow the recommended timing strictly to ensure consistency in results.

4. Excessive washing force or too many washes. Solution: Wash 4–5 times with 250 µL per well, and gently pat dry after each step.

5. Exposure to direct sunlight or air flow. Solution: Avoid placing the plate under direct light or in a drafty area during the process.

6. Incorrect wavelength setting on the microplate reader. Solution: Ensure the reader is set to 450 nm for ELISA measurements.

7. Mistakes in antibody dilution. Solution: Double-check your dilution ratios and use a calibrated pipette for accuracy.

8. Poor water quality. Solution: Use high-quality water such as purified or mineral water (e.g., Wahaha) for reagent preparation.

In conclusion, every experiment comes with its challenges, but with the right approach and tools, you can achieve reliable and reproducible results. At Shanghai Guduo Biotechnology, we believe that choosing the right equipment and following best practices can significantly improve your experimental outcomes. Whether it's optimizing your budget or reducing time spent on troubleshooting, understanding the cost-benefit ratio of your experiments is essential. The better the return on your investment, the more efficient your research will be. Always consider your available resources and plan accordingly for the best results.